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为研究麦冬类植物材料的遗传多样性,利用非变性聚丙烯酰胺凝胶电泳技术,用8对EST-SSR引物在32份麦冬类植物材料进行扩增检测,并对样本进行聚类分析。结果表明:8对引物的PIC(Polymorphic information content)位于0~0.869,平均值0.567,引物1扩增出最多的等位基因数,占总值的20.41%;遗传距离矩阵研究发现,个体1和个体23间的遗传距离最大为2.48,说明二者遗传分化程度最大,个体18和个体23地理来源虽然不同,但二者间遗传距离最小,表明二者间有一定程度的基因交流现象;同时,居群c(野生杭麦冬)内的平均遗传距离最小为0.03,居群d(阔叶山麦冬)内的平均遗传距离最大为0.93。在聚类分析中,32份材料被分为2大类,在主坐标分析中,32份材料被分为3大类,2种分析结果具有相似性。研究表明EST-SSR标记可以很好地对麦冬类植物材料进行划分。
In order to study the genetic diversity of Ophiopogon japonicus, 8 pairs of EST-SSR primers were used to amplify 32 Ophiopogon japonicus plants by using non-denaturing polyacrylamide gel electrophoresis, and the samples were clustered . The results showed that PIC (Polymorphic information content) of 8 pairs of primers was located at 0 ~ 0.869 with an average of 0.567, and the number of alleles amplified by primer 1 was the highest, accounting for 20.41% of the total. Genetic distance matrix showed that individuals 1 and The genetic distance between individuals 23 was 2.48, indicating that the two had the highest degree of genetic differentiation. Although the geographical origins of individuals 18 and individuals 23 were different, the genetic distance between them was the smallest, indicating that there was a certain degree of gene exchange between the two. At the same time, The average genetic distance within population c (wild Hokkaido) was 0.03 at the minimum, and the average genetic distance within population d (Ophiopogon japonicus) was 0.93. In the cluster analysis, 32 materials were divided into two categories. In the main coordinate analysis, 32 materials were divided into three categories, and the two kinds of analysis results were similar. Studies have shown that EST-SSR markers can be well divided Ophiopogon plant material.