论文部分内容阅读
目的:探讨p21及TGF-β在肾间质纤维化中的作用及其可能的调控机制。方法:将SD大鼠随机分为假手术组(SOR组)和单侧输尿管结扎组(UUO组)。HE染色观察2组大鼠术后第7,14和21天肾小管间质的形态学改变;用RT-PCR法检测肾皮质p21 mRNA和TGF-βmRNA的变化。结果:在UUO大鼠模型早期,肾皮质p21 mRNA和TGF-βmRNA的表达即有明显增高,与SOR组大鼠比较,差异有显著性(分别P<0.01及P<0.05),随着梗阻时间的延长,p21和TGF-β的表达逐渐增强(P<0.05),并且UUO组大鼠各时间点p21和TGF-β的表达呈正相关(各时间点分别为r=0.745、0.700、0.662,P<0.05)。结论:p21可能参与了单侧输尿管梗阻大鼠肾小管间质纤维化的发病过程,TGF-β可能参与介导了p21在肾皮质表达的调控。
Objective: To investigate the role of p21 and TGF-β in renal interstitial fibrosis and its possible regulatory mechanism. Methods: SD rats were randomly divided into sham operation group (SOR group) and unilateral ureteral ligation group (UUO group). Morphological changes of tubulointerstitium were observed on the 7th, 14th and 21th days in the two groups by HE staining. The changes of p21 mRNA and TGF-βmRNA in renal cortex were detected by RT-PCR. Results: The expressions of p21 mRNA and TGF-βmRNA in renal cortex were significantly increased in UUO rat model at early stage (P <0.01 and P <0.05, respectively) compared with those in SOR group. With the increase of obstruction time , The expression of p21 and TGF-β gradually increased (P <0.05), and there was a positive correlation between the expression of p21 and TGF-β at each time point in UUO group (r = 0.745,0.700,0.662, P <0.05). Conclusion: p21 may be involved in the pathogenesis of tubulointerstitial fibrosis in rats with unilateral ureteral obstruction. TGF-β may be involved in the regulation of p21 expression in the renal cortex.