论文部分内容阅读
目的：检测５４例胃癌切除标本的癌组织中ｒａｓｐ２１表达、癌细胞核形态参数和ＤＮＡ含量及倍体分布类型。方法：采用组织化学、免疫组织化学和自动图像分析方法。结果：发现胃癌细胞ｐ２１表达水平随着ＤＮＡ含量不断增多、倍体成倍增加而逐渐下降。表明ｒａｓｐ２１过度表达主要出现在细胞表型发生明显转化之前。结论：胃癌细胞ｐ２１的表达水平与癌细胞核形态参数、癌肿大小、有无淋巴结转移均无关。
OBJECTIVE: To detect the expression of rasp21, nuclear morphological parameters, DNA content, and type of ploidy in cancer tissues from 54 specimens of gastric cancer. Methods: Histochemistry, immunohistochemistry, and automated image analysis methods were used. Results: The expression level of p21 in gastric cancer cells was gradually decreased as the DNA content increased and the ploidy increased exponentially. It was suggested that the overexpression of rasp21 mainly occurs before the cell phenotype has undergone significant transformation. Conclusion: The expression level of p21 in gastric cancer cells is not related to the nuclear morphological parameters, cancer size, and lymph node metastasis.