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目的探讨纤溶酶原激活剂抑制物-1(Plasminogen activator inhibitor-1,PAI-1)基因启动子区-675bp处4G/5G多态性与潍坊地区汉族儿童哮喘发病的易感性及其临床特点的关系。方法采用病例-对照研究方法,选取196例哮喘患儿(儿童哮喘组)和173例健康儿童(对照组)为研究对象,应用限制性片段长度多态性(PCR-RFLP)分析方法检测PAI-1基因4G/5G多态性。结果儿童哮喘组和对照组之间,PAI-1基因3种基因型(4G/4G、4G/5G、5G/5G)频率分布差异有统计学意义(P<0.01)。4G/4G基因型频率(28.0%)显著高于对照组(14.4%;P<0.01);与5G/5G基因型比较,携带4G/4G型儿童发生哮喘的相对风险(odds ratios,OR)增加2.95倍(95%CI:1.58~5.50;P<0.01)。携带4G等位基因儿童发生哮喘的OR是5G等位基因型个体的1.61倍(95%CI:1.20~2.15;P<0.01)。儿童哮喘组PAI-1基因不同基因型之间Ig E、CRP、LYM、EOS、NEU检测结果比较,差异均无统计学意义(P>0.05)。结论 PAI-1基因4G/5G多态性与潍坊地区儿童哮喘的易感性相关,4G/4G型为易感基因型;该位点多态性与儿童哮喘Ig E、CRP、LYM、EOS、NEU等指标无相关性。
Objective To investigate the polymorphisms of 4G / 5G at -675bp in promoter region of Plasminogen activator inhibitor-1 (PAI-1) gene and the susceptibility and clinical features of asthma in Han children in Weifang Relationship. Methods A case-control study was conducted in 196 asthmatic children (asthmatic children with asthma) and 173 healthy children (control group) as study subjects. PCR-RFLP was used to detect PAI- 1 gene 4G / 5G polymorphism. Results There was significant difference in the frequency distribution of PAI-1 genotype (4G / 4G, 4G / 5G, 5G / 5G) between childhood asthma group and control group (P <0.01). The frequency of 4G / 4G genotype (28.0%) was significantly higher than that of the control group (14.4%; P <0.01). Compared with the 5G / 5G genotype, the odds ratios (OR) 2.95-fold (95% CI: 1.58-5.50; P <0.01). The odds of developing asthma in children with the 4G allele were 1.61 times those in the 5G allele (95% CI: 1.20-2.15; P <0.01). There was no significant difference in IgE, CRP, LYM, EOS and NEU among different genotypes of PAI-1 gene in children with asthma (P> 0.05). Conclusion 4G / 5G polymorphism of PAI-1 gene is associated with susceptibility to asthma in children in Weifang region. 4G / 4G genotype is predisposing genotype. Polymorphism of this polymorphism is associated with IgE, CRP, LYM, EOS, NEU No correlation between other indicators.