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用AFLP分子标记技术,对花粉管介导玉米总DNA获得的2个高秆水稻变异植株C(HN28)和D(HN31)进行分析,揭示水稻变异植株和亲本间在DNA水平的差异,为进一步研究变异机制提供数据。首先对64对AFLP选扩引物进行筛选,优选出11对选扩引物,它们均能够在2个变异植株中扩增出较多清晰的DNA差异带。对变异植株和对照中的DNA图谱进行分析,结果显示,变异植株C、D、受体水稻B与阳性对照玉米A之间的DNA图谱相似率分别为38.30%、38.58%和32.99%,水稻变异植株C、D和受体水稻B的DNA图谱相似率分别为90.04%和85.84%,说明变异植株与受体水稻基因组DNA存在显著差异,变异植株与阳性对照玉米基因组DNA相似率明显高于受体水稻。变异植株C检测到12条与对照玉米一致的目的带,变异植株D检测到11条与对照玉米一致的目的带。说明变异植株C、D基因组中可能存在有玉米DNA片段,变异性状的差异可能是介导玉米DNA引起。
AFLP molecular marker technique was used to analyze the differences of DNA level between two mutant rice C (HN28) and D (HN31) obtained from pollen tube-mediated maize total DNA. Research mutation mechanism to provide data. First, 64 pairs of AFLP selection primers were screened, and 11 pairs of selection primers were selected, each of which could amplify more distinct DNA difference bands in two mutant plants. The DNA profiles of the mutants and controls were analyzed. The results showed that the similarity of the DNA profiles of C, D, Receptor B and positive control A were 38.30%, 38.58% and 32.99%, respectively. The similarities of the DNA profiles of C, D and B were 90.04% and 85.84%, respectively, indicating that there was a significant difference between the genomic DNA of the mutant plants and that of the recipient plants. The similarity of genomic DNA between the mutant plants and the positive control maize was significantly higher than that of the recipient Rice. Mutant C detected 12 target maize lines consistent with the control maize, and mutant D detected 11 matings consistent with the control maize. The results indicated that there may exist DNA fragments of maize in the C and D genomes of the mutant plants. The differences of the variation traits may be caused by the mediation of maize DNA.