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目的 研究CD5分子对套细胞淋巴瘤细胞株Jeko-1的化疗敏感性及其通路的影响。方法 Crispr/cas9法敲除人源套细胞淋巴瘤细胞株Jeko-1(P)CD5基因靶位点,构建CD5阴性Jeko-1(N)细胞。RT-PCR检测Jeko-1(P)与Jeko-1(N)中CD5-E1A与CD5-E1B的表达。MTS法检测柔红霉素、长春新碱对Jeko-1(P)与Jeko-1(N)细胞增殖抑制效应。ELISA法检测Jeko-1(P)与Jeko-1(N)中IL-10表达。Western blot法检测BAY 11-7