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研究牛磺酸对晶体氧化损伤的保护作用。方法 :将正常兔晶体分为正常条件培养组 (对照组 ) ,Feton模型组 (氧化损伤组 ) ,氧化损伤同时给 0 .5%和 1 %牛磺酸保护组。体外培养 2 4 h后 ,检测各组晶体的总抗氧化能力 (T- AOC)水平 ,可溶性蛋白 (sol- prot)含量 ,超氧化歧化酶 (SOD) ,过氧化氢酶 (CAT)及谷胱苷肽过氧化物酶 (GSH- PX)活性 ,以及脂质过氧化反应产物丙二醛 (MDA)含量。结果 :和对照组比较 ,Feton模型组晶体 T- AOC水平 ,SOD、CAT和 GSH- PX活性 ,slo- prot含量明显降低 ,MDA含量明显升高 ;和 Feton模型组相比 ,牛磺酸保护组晶体 T- AOC、SOD、CAT、GSH- PX、sol- prot明显升高 ,MDA明显下降 ,以 1 %牛磺酸组最为明显。结论 :牛磺酸有保护体外培养的兔晶体免受氧化损伤 ,抑制其脂质过氧化的作用。
To study the protective effect of taurine on crystal oxidative damage. Methods: The normal rabbits were divided into normal culture group (control group), Feton model group (oxidative damage group) and oxidative damage group (0. 5% and 1% taurine). Twenty-four hours later, T-AOC, sol-prot, SOD, CAT, Glycoside peroxidase (GSH-PX) activity, and lipid peroxidation product malondialdehyde (MDA) content. Results: Compared with the control group, the levels of T-AOC, the activities of SOD, CAT and GSH-PX, the content of slo-prot and the content of MDA in Feton model group were significantly higher than those in Feton model group The contents of T-AOC, SOD, CAT, GSH-PX, and sol-prot were significantly increased, MDA significantly decreased, and the most obvious was in 1% taurine group. Conclusion: Taurine can protect rabbit crystal from oxidative damage and inhibit its lipid peroxidation.