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本文报导了用聚丙烯酞胺凝胶垂直平板电泳法,分析青海省同一地区不同品种(系)和不同地区同一品种(系)春小麦的过氧化物酶(POD)同工酶,发现同一地区不同品种(系)的春小麦的过氧化物酶同工酶谱差异显著,并具有自己的特征谱带;不同地区同品种春小麦的酶谱基本相似。说明遗传物质的组成是决定过氧化物酶同工酶的基础,它的表达基本不受地理环境和栽培技术的影响,故该技术可作为品种鉴定的生化依据之一。
This paper reports the analysis of peroxidase (POD) isozymes in spring wheat cultivars with the same variety (line) and in different areas in the same area of Qinghai by polyacrylamide gel electrophoresis and vertical plate electrophoresis. The varieties of spring wheat had significantly different peroxidase isozymes and had their own characteristic bands. The enzyme patterns of spring wheat in different regions were similar. It shows that the composition of genetic material is the basis for determining peroxidase isoenzyme. Its expression is basically not affected by the geographical environment and cultivation techniques, so the technology can be used as one of the biochemical basis for identification of varieties.