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目的探讨运动性疲劳后脊髓前角5-羟色胺(5-hydroxytryptamine,5-HT)及其合成关键酶色胺酸羟化酶(tryptophan hydroxylase,TPH)表达的变化,阐释在脊髓水平运动性疲劳的发生机制。方法健康成年雄性Wistar大鼠16只随机分为运动性疲劳组和对照组,采用免疫组化方法和计算机图像分析技术,检测大鼠脊髓前角的5-HT及TPH免疫反应阳性纤维及终末的平均积分光密度值。结果运动性疲劳后,脊髓前角5-HT及TPH阳性反应纤维和终末减少,染色较浅,平均积分光密度值明显低于对照组(P<0.05)。结论脊髓前角5-HT及TPH的含量在运动性疲劳后降低,提示脊髓前角5-HT与运动性疲劳的发生有关。
Objective To investigate the expression of 5-hydroxytryptamine (5-HT) and its key enzyme of tryptophan hydroxylase (TPH) after exercise-induced fatigue in rats, The mechanism. Methods Sixteen healthy adult male Wistar rats were randomly divided into exercise-induced fatigue group and control group. Immunohistochemistry and computer image analysis were used to detect the expression of 5-HT and TPH immunoreactive fibers in the anterior horn of spinal cord and the terminals Average integrated optical density value. Results After exercise fatigue, the positive fibers and terminals of 5-HT and TPH decreased in the anterior horn of spinal cord. The average staining optical density was significantly lower than that of the control group (P <0.05). Conclusions The contents of 5-HT and TPH in the anterior horn of spinal cord decrease after exercise-induced fatigue, suggesting that 5-HT in spinal cord anterior horn is associated with the occurrence of exercise-induced fatigue.