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对小鼠睾丸冰冻切片ＬＤＨ－Ｘ的显示方法作了进一步探讨。实验表明，以Ｌ－２－羟基－３－甲基戊酸（ＨＭＶ）为特异性底物，能相当有效地显示睾丸ＬＤＨ－Ｘ的分布，且该底物的配制方法简便易行。在显示ＬＤＨ－Ｘ的孵育液中采用萘酚蓝（ＮＢ）作中间递电子体较吩嗪甲基硫酸酯更具优越性。
The mouse testes frozen section LDH-X display method to further explore. Experiments show that L-2-hydroxy-3-methyl-valeric acid (HMV) as a specific substrate, can be quite effective in the testis LDH-X distribution, and the preparation of the substrate method is simple and easy. The use of naphthol blue (NB) as the intermediate electron donor in the incubation of LDH-X shows more advantages than phenazine methyl sulfate.