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目的 探讨国产罗格列酮对高糖高脂喂养诱导树肝脂肪变性干预治疗的效果。方法 36只树分为对照组 8只 (A组 ) ,喂常规鼠饲料。另 2 8只喂高糖高脂饲料 ,并于 2 2周将血糖≥ 11.1mmol/L的 19只动物再分为药物干预组 8只 (B组 )和非干预组 11只 (C组 )。B组以国产罗格列酮按每天 5mg/kg的剂量清晨一次性给药 ,用药 7周。实验前、后检测体重 (Wt)、空腹血糖 (FBG)、血脂 4项[总胆固醇 (TC) ,三酰甘油 (TG) ,高密度脂蛋白胆固醇 (HDL C) ,低密度脂蛋白胆固醇 (LDL C) ]、空腹血清胰岛素 (FINS)和血清转氨酶水平。实验结束时剖腹取肝标本 ,光镜下观察肝脏形态学改变 ,以免疫组化法检测肝脏解偶联蛋白 3(UCP3)和胰岛素受体 β(IR β)的表达水平 ,以计算机图像分析系统对UCP3、IR β表达作细胞阳性计数和面积密度及灰度分析。 结果 (1)血生化改变 :B、C两组动物FBG均明显增高 ,实验结束时C组的血糖仍居高不下 ,同时血TC、TG增加值、FINS和自身稳定模型评估法胰岛素抵抗指数 (HOMA IR)也明显增高 ,而B组的FBG则显著下降 ,其他生化参数也均与对照组相似。 (2 )肝脏形态学 :B、C两组动物均出现弥漫性肝脂肪变性 ,脂肪变性的面积密度和肝细胞脂变率均在 30 %左右 ,两组差异均无显著性。实验结束时 ,B组肝脂?
Objective To investigate the effect of domestic rosiglitazone on hepatic steatosis induced by high glucose and high fat diet in rats. Methods Thirty-six tree shrews were divided into control group (group A) and normal rat feed. The other 28 rabbits were fed with high-fat and high-fat diet. In the 22 weeks, 19 animals with blood glucose> 11.1 mmol / L were further divided into 8 groups (group B) and 11 non-intervention group (group C). Group B domestic rosiglitazone 5mg / kg daily dose of a single morning administration, medication for 7 weeks. Body weight (Wt), fasting blood glucose (FBG), lipid 4 (total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDL C), low density lipoprotein cholesterol C)], fasting serum insulin (FINS) and serum transaminase levels. At the end of the experiment, the liver specimens were taken by laparotomy. The morphological changes of the liver were observed under light microscope. The expression levels of uncoupling protein 3 (UCP3) and insulin receptor beta (IRβ) in the liver were detected by immunohistochemical method and analyzed by computer image analysis system UCP3, IR β expression for cell-positive count and area density and grayscale analysis. Results (1) The blood biochemical changes: The FBG of both groups were significantly higher than that of the control group (P <0.05). At the end of the experiment, the blood glucose in C group was still high while the TC, TG, FINS and insulin resistance index HOMA IR) were also significantly higher, while FBG was significantly decreased in group B, other biochemical parameters are also similar to the control group. (2) Morphology of liver: Diffuse hepatic steatosis occurred in both groups B and C, and the area density of steatosis and hepatocellular fatty degeneration were all about 30%. There was no significant difference between the two groups. At the end of the experiment, group B hepatic lipid?