目的 :建立高灵敏和高特异性的IL 8放射免疫分析方法。方法 :用人工重组的白细胞介素 8(IL 8)多次免疫兔和豚鼠 ,获取高效价的IL 8抗体。用氯氨T法制备1 2 5 I标记IL 8,经SephadexG 2 5纯化 ,抗原抗体反应采用平衡一步法 ,4℃培养2 4h后经PR试剂分离结合和游离的标记抗原。结果 :该法测定范围 0 .2～ 9.6ng ml,最低检出量为 0 .1ng ml,批内和批间误差分别小于 5 %和 10 %。正常人血清含量为 (0 .35± 0 .0 1)ng ml(n =6 4)。人粒细胞系HL 6 0细胞体外培养 2 4h ,钙离子载体A2 31 87和内毒素脂多糖 (LPS)可显著提高细胞释放IL 8,而蛋白激酶C激活剂 (PMA)却没有明显的促IL 8释放作用。另外 ,兔失血性休克再灌注损伤后 2 4h内不同时间血清中IL 8水平明显高于对照。结论 :该方法是一种简便、灵敏和特异的IL 8分析方法 ,可用于人和兔血清以及细胞培养上清液中IL 8分析 Objective: To establish a highly sensitive and specific IL 8 radioimmunoassay. Methods: Rabbit and guinea pigs were immunized with recombinant interleukin 8 (IL 8) several times to obtain high titer IL 8 antibody. The IL-8 labeled with 125 I was prepared by chloramine T method and purified by SephadexG 2 5. The antigen-antibody reaction was performed by equilibrium one-step method and incubated at 4 ° C for 24 h. The bound and free labeled antigens were separated by PR reagent. Results: The method has a range of 0.2-2.6ng ml and a minimum detectable volume of 0.1ng ml. The in-batch and inter-batch errors are less than 5% and 10% respectively. Normal serum (0. 35 ± 0. 01) ng ml (n = 6 4). The human granulocyte cell line HL 60 was cultured for 24 h in vitro. Calcium ionophore A2 31 87 and lipopolysaccharide (LPS) significantly increased the release of IL-8 from cells, whereas the protein kinase C activator (PMA) did not induce IL 8 release effect. In addition, the levels of IL-8 in serum at 24 hours after hemorrhagic shock-reperfusion injury were significantly higher than those in controls. Conclusion: This method is a simple, sensitive and specific method for the analysis of IL-8 and can be used for IL-8 analysis in human and rabbit serum as well as in cell culture supernatants