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用琼脂平板法和单向免疫扩散法分别测定唾液溶菌酶和唾液IgG、IgA;用1%重铬酸钾溶液作皮肤斑贴试验;用分光光度法测尿铬。结果:直接接触组唾液溶菌酶含量明显低于经口摄入组及对照组(P<0.01);而唾液IgG、IgA均明显高于后两组(P<0.01);皮肤斑贴试验阳性率亦明显高于后两组(P<0.05,P<0.01);直接接触组和经口摄入组尿铬水平均明显高于对照组(P<0.01)。结果提示:铬经呼吸道进入可影响机体免疫功能,但经消化道长期低剂量摄入对人体免疫功能影响不大。
Salivary lysozyme and saliva IgG, IgA were determined by agar plate method and unidirectional immunodiffusion method respectively; skin patch test was conducted with 1% potassium dichromate solution; urine chromium was measured by spectrophotometry. Results: The content of salivary lysozyme in direct contact group was significantly lower than that in oral intake group and control group (P <0.01), while salivary IgG and IgA in saliva were significantly higher than those in the latter two groups (P <0.01) Also significantly higher than the latter two groups (P <0.05, P <0.01). The levels of urinary chromium in the direct contact group and the oral ingestion group were significantly higher than those in the control group (P <0.01). The results suggest that: chromium enters the respiratory tract can affect the body’s immune function, but long-term low-dose intake of the digestive tract on human immune function has little effect.