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本文建立了测定家兔血浆中Ｚ－４７（３Ｈ－１，２－二氢－２－（４－甲基苯胺基）甲基－１－吡咯里嗪酮）的高效液相色谱法。选用μ－ＢｏｎｄａｐａｋＣ１８色谱柱，流动相为甲醇－磷酸二氢铵缓冲液（ｐＨ＝４）（３：２，Ｖ／Ｖ）。以安定为内标物。血浆样品先经碱化，用乙醚－正己烷（１：１，Ｖ／Ｖ）提取，分离有机相，再以１０％磷酸萃取，水相２０μＬ进样，紫外检测波长２９０ｎｍ。本法专属性强，精密度、准确性符合有关要求，操作简便。对６只家兔口服给药后应用本法成功地进行了初步的药代动力学研究。
In this paper, high performance liquid chromatography (HPLC) was developed for the determination of Z-47 (3H-1,2-dihydro-2- (4-methylanilino) methyl-1-pyrrolizinone in rabbit plasma. The μ-Bondapak C18 column was used and the mobile phase was methanol-ammonium dihydrogen phosphate buffer (pH = 4) (3: 2, V / V). To stability as an internal standard. The plasma samples were alkalized and extracted with diethyl ether-n-hexane (1: 1, V / V). The organic phase was separated and extracted with 10% phosphoric acid. The aqueous phase was injected with 20 μL and the UV detection wavelength was 290 nm. This law is unique, precision, accuracy in line with the relevant requirements, easy to operate. After oral administration to 6 rabbits, preliminary pharmacokinetic studies were successfully performed using this method.