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目的建立HPLC法同时测定新疆贝母中3种核苷类成分的含有量。方法贝母水提液的分析采用Waters Sun Fire TM-C_(18)色谱柱(4.6 mm×150 mm,5μm);流动相甲醇-水(10∶90),等度洗脱;体积流量0.8 m L/min;柱温25℃;检测波长260 nm。结果尿苷、胸苷、腺苷分别在10.04~501.93μg/m L(r=0.997 5)、0.10~5.07μg/m L(r=0.999 8)、1.01~50.39μg/m L(r=0.999 9)范围内呈良好的线性关系,平均加样回收率分别为103.11%(RSD=1.45%)、99.33%(RSD=1.68%)、98.25%(RSD=1.57%)。结论该方法简便准确,灵敏度高,专属性好,可用于新疆贝母的质量控制。
OBJECTIVE To establish an HPLC method for the simultaneous determination of three nucleosides in Fritillaria thunbergii. Methods The aqueous extract of Fritillaria cirrhosae was separated on a Waters Sun Fire TM-C 18 column (4.6 mm × 150 mm, 5 μm) using a mobile phase of methanol-water (10: 90) L / min; column temperature 25 ℃; detection wavelength 260 nm. Results The results showed that uridine, thymidine and adenosine levels were significantly higher in the range of 10.04 ~ 501.93μg / m L (r = 0.997 5), 0.10 ~ 5.07μg / m L (r = 0.999 8) and 1.01 ~ 50.39μg / m L 9). The average recoveries were 103.11% (RSD = 1.45%), 99.33% (RSD = 1.68%) and 98.25% (RSD = 1.57%), respectively. Conclusion The method is simple, accurate, sensitive and specific. It can be used for the quality control of Xinjiang Fritillaria.