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目的探索甲型病毒性肝炎患者外周血淋巴细胞糖皮质激素受体（ＧＲ）的表达与血清中皮质醇激素浓度的相互关系。方法分别对部分正常人和３２例急性甲型肝炎患者淋巴细胞内ＧＲ表达状况进行了流式细胞仪测定，同时应用放射免疫分析法测定其血清皮质醇含量。结果甲型肝炎患者在急性期其血清皮质醇含量显著高于正常对照组，分别为２１０±７６μｇ／Ｌ，１４２±８３μｇ／Ｌ（患者组与正常人组比较Ｐ＜０．０１）；患者淋巴细胞内的ＧＲ含量则较正常人群明显降低。以本研究的实测值为标准，正常人ＧＲ阳性淋巴细胞为１４．２％±６．８８％，而患者组降低至８．５％±４．０７％，ＧＲ表达与血清皮质醇含量间呈明显的负相关。结论血清皮质醇水平与ＧＲ受体间存在对应关系并可能与患者的免疫状态与病情密切相关。
Objective To explore the relationship between the expression of glucocorticoid receptor (GR) in peripheral blood lymphocytes and serum cortisol levels in patients with viral hepatitis A (A). Methods The expression of GR in lymphocytes of some normal subjects and 32 patients with acute hepatitis A were measured by flow cytometry. The serum cortisol level was measured by radioimmunoassay. Results The level of serum cortisol in patients with acute hepatitis A was significantly higher than that of the controls (210 ± 76μg / L, 142 ± 83μg / L, P <0.01, P <0.01) Intracellular GR content was significantly lower than the normal population. Based on the measured values of this study, the normal human GR-positive lymphocytes were 14.2% ± 6.88%, while the patients were reduced to 8.5% ± 4.07%. The expression of GR was positively correlated with serum cortisol Obvious negative correlation. Conclusion There is a corresponding relationship between serum cortisol level and GR receptor and may be closely related to the patient’s immune status and disease.