在蛋白质组学研究中首先要应用各种分离技术如双向电泳等将蛋白分离纯化,然后结合质谱技术、生物信息学方法对蛋白质进行鉴定。但细胞内的蛋白质种类繁多,性质多样,丰度差异大,常规蛋白质组学方法不能做到分离和检测细胞中的全部蛋白质[1 ] 。亚细胞蛋白质组学研究的着眼点在蛋 Proteomics in the first study to apply a variety of separation techniques such as two-dimensional electrophoresis separation and purification of proteins, and then combined with mass spectrometry, bioinformatics methods for protein identification. However, a wide range of intracellular proteins, diverse nature, abundance differences, conventional proteomics methods can not do the separation and detection of all cells in the protein [1]. Subcellular proteomics research focuses on the egg