论文部分内容阅读
目的:评价吡氟酰草胺原药对大鼠睾丸的损伤效应及其机制,为完善该新型除草剂的安全性评价及防护措施提供实验依据。方法:分别给予雄性SD大鼠0、20(低)、100(中)和500 mg/kg/d(高)的吡氟酰草胺连续经口染毒90天,观察其一般毒性表现;测定血清中谷胱甘肽(GSH)、总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)的含量;评价大鼠的睾丸损伤,采用免疫印迹法检测睾丸组织中Cleaved-caspases 3、C-PRAR、FAS/FASL等蛋白的表达,并运用免疫组化法进行验证。结果:与对照大鼠相比,中、高剂量组的吡氟酰草胺能明显降低大鼠的体重增长及食物利用率(P<0.05);血清SOD和T-AOC水平下降,以高剂量组SOD下降显著(P<0.05),但GSH未见变化;染毒大鼠睾丸的生精小管空泡化严重、管腔内精原细胞显著减少;低、高剂量组大鼠睾丸组织中Cleaved-caspases 3、C-PRAR、FAS的表达显著增加;免疫组化也证实,低、高剂量组染毒大鼠生精小管中Cleaved-caspases 3阳性的细胞数显著增加(P<0.05)。结论:吡氟酰草胺可引起大鼠睾丸中的抗氧化能力下降,睾丸可能是该除草剂潜在的靶器官,氧化应激通路可能参与了吡氟酰胺所致的雄性生殖毒性。
OBJECTIVE: To evaluate the damaging effect of difluralide on rat testes and its mechanism, and to provide experimental evidence for improving the safety evaluation and protective measures of this novel herbicide. Methods: The rats were treated with 0, 20 (low), 100 (medium) and 500 mg / kg / d (high) of difloxacin for 90 consecutive days in male SD rats. The general toxicity was observed. Serum glutathione (GSH), total antioxidant capacity (T-AOC), and superoxide dismutase (SOD) were measured. The testicular injury was evaluated. Cleaved-caspases 3 C-PRAR, FAS / FASL protein expression, and using immunohistochemistry to verify. Results: Compared with the control rats, midazolam could significantly reduce the body weight gain and food utilization (P <0.05), and the levels of serum SOD and T-AOC in the middle and high dose groups decreased significantly (P <0.05), but there was no change in GSH; the seminiferous tubules in the testis of rats were severely vacuolized and the number of spermatogonia in the lumen was significantly reduced; the levels of Cleaved -caspases 3, C-PRAR and FAS. Immunohistochemistry also showed that the numbers of Cleaved-caspases 3 positive cells in the low and high dose groups were significantly increased (P <0.05). CONCLUSION: Diffluramide can reduce the antioxidant capacity of testis in rats, and testis may be a potential target organ for this herbicide. The oxidative stress pathway may be involved in the reproductive toxicity of pyripamide.