通过比较SDS和CTAB 2种方法 ,CTAB法更适于油茶DNA的提取 ,并在CTAB法提取过程中将一些有效的去多糖、多酚的步骤进行组合 ,建立适合油茶DNA的提取方法 ,同时构建了油茶DNA的PCR扩增程序 ,并从预变性温度、Mg2 +浓度、dNTPs、TaqDNA聚合酶、模板DNA等方面确定了PCR扩增条件 By comparing SDS and CTAB two methods, CTAB method is more suitable for the extraction of Camellia oleifera DNA, and in the CTAB extraction process will be some effective polysaccharide, polyphenol steps to be combined to establish a suitable extraction method Camellia DNA, while building The PCR amplification procedure of camellia DNA was established and the PCR amplification conditions were determined from the aspects of the denaturation temperature, Mg2 + concentration, dNTPs, Taq DNA polymerase and template DNA