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目的:观察多西他赛对人胃癌裸鼠移植瘤的放射增敏作用和多西他赛联合放射对肿瘤细胞凋亡的影响。方法:建立人AGS胃癌裸鼠移植瘤动物模型28只,随机分为4组:对照组、单纯照射组、单纯多西他赛组、多西他赛+照射组。测量各组移植瘤体积,计算各组抑瘤率,评价多西他赛的放射增敏效果,并对标本进行光镜观察及原位末段标记(TUNEL)检测。结果:28只裸鼠的成瘤率为100%,实验期间无死亡,比较各组移植瘤体积差异有统计学意义(P<0.01)。各组抑瘤率分别为单纯照射组36.4%,单纯多西他赛组44.2%,多西他赛+照射组86.9%。多西他赛对放射治疗的增敏值(enhancement factor,EF)值为1.5。多西他赛+照射组的平均凋亡指数(AI)为(15.6±2.3)%,与其它各组比较差异有统计学意义(P<0.01)。结论:多西他赛对人AGS胃癌裸鼠移植瘤有良好的放射增敏作用,多西他赛联合放疗能更有效地诱导肿瘤细胞凋亡。
OBJECTIVE: To observe the radiosensitization effect of docetaxel on human gastric cancer xenografts in nude mice and the effect of docetaxel combined with radiotherapy on tumor cell apoptosis. Methods: Totally 28 animal models of AGS in nude mice were established and randomly divided into 4 groups: control group, simple irradiation group, docetaxel group and docetaxel + irradiation group. The tumor volume of each group was measured. The tumor inhibition rate of each group was calculated. Radiosensitization effect of docetaxel was evaluated. The specimens were examined by light microscopy and TUNEL. Results: The tumor formation rate of 28 nude mice was 100%, and there was no death during the experiment. There was significant difference in the volume of xenograft between the two groups (P <0.01). The inhibition rate of each group was 36.4% in the simple irradiation group, 44.2% in the simple docetaxel group and 86.9% in the docetaxel + irradiation group. Docetaxel had an enhancement factor (EF) value of 1.5 for radiotherapy. The average apoptotic index (AI) in docetaxel + irradiation group was (15.6 ± 2.3)%, which was significantly different from other groups (P <0.01). Conclusion: Docetaxel has a good radiosensitization effect on human gastric cancer xenografts in nude mice, and docetaxel combined with radiotherapy can induce tumor cell apoptosis more effectively.