采用原位氧化复合法制备了聚苯胺/金纳米纤维,并将其固载在玻碳电极上构建了一种新型的过氧化氢生物传感器。该传感器对过氧化氢的还原具有良好的催化活性,在优化的实验条件下,该传感器对过氧化氢测定的线性范围为1.010-6～1.0510-4mol/L,检出限为5.7510-7mol/L(S/N=3)。 Polyaniline / gold nanofibers were prepared by in situ oxidation complex method and immobilized on glassy carbon electrode to construct a new type of hydrogen peroxide biosensor. The sensor has good catalytic activity for the reduction of hydrogen peroxide. Under the optimal experimental conditions, the sensor has a linear range of 1.010-6 ~ 1.0510-4mol / L for hydrogen peroxide with a detection limit of 5.7510-7mol / L (S / N = 3).