目的寻求食物中毒诊断及食品检测的有效方法。方法建立多重PCR体系一次检测多种食品致病菌方法。结果可以一次检测出肠毒性大肠埃希菌(E.coli-ETEC),蜡样芽胞杆菌(Bacilluscereus),沙门菌属(Salmonellaspp),大肠埃希菌O157:H7(E.coli-O157:H7),志贺菌属(Shigellaspp),霍乱弧菌(Vibriocholerae)等菌属或菌。结论多重PCR体系检测6种菌DNA的灵敏度最低为10.30fg,与单独的PCR检测的灵敏度相同。将它做成体外基因诊断试剂盒,将成为细菌性食物中毒诊断及食品检测的有效工具。 Objective To seek food poisoning diagnosis and food testing an effective method. Methods To establish a multiplex PCR system for the detection of multiple pathogenic bacteria in food at a time. As a result, E. coli-ETEC, Bacillus cereus, Salmonellaspp, Escherichia coli O157: H7 (E. coli-O157: H7) Shigellaspp, Vibriocholerae and the like. Conclusion The sensitivity of multiplex PCR system for detection of six kinds of bacteria DNA is 10.30fg, which is the same as that of PCR assay alone. Making it an in vitro genetic diagnostic kit will be an effective tool for the diagnosis and food detection of bacterial food poisoning.