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目的 研究α-黑色素细胞刺激素(α-MSH)对急性失血性休克合并肺内毒素损伤两次打击致急性呼吸窘迫综合征大鼠血清细胞因子水平的影响。方法 30只雄性SD大鼠随机分成生理对照组(A组)、急性呼吸窘迫综合征组(B组)和给药组(C～F组,在不同时间点静脉注射α-MSH 1.7 mg·kg-1)。观察各组血清细胞因子(TNF-α、IL-1、IL-6、IL-10)水平的变化。结果 生理对照组(A组)大鼠血清细胞因子水平各时间点间差异无显著性(P>0.05);急性呼吸窘迫综合征组(B组)大鼠随打击因素和病程进展,血清细胞因子TNF-α、IL-1、IL-6、IL-10水平均显著增高(P<0.01),促/抗炎细胞因子平衡失衡;给药组(C、D、F组)与B组相比能显著降低血清TNF-α、IL-1水平(P<0.01),增加IL-6、IL-10浓度(P<0.01);其中多次给药组(F组)作用最明显,而内毒素致伤后1 h给药组(E组)各细胞因子水平与B组各时间点相比差异无显著性(P>0.05)。结论 α-MSH可降低急性呼吸窘迫综合征大鼠血清促炎细胞因子水平促进抗炎细胞因子产生。α-MSH可能通过调节促/抗炎细胞因子平衡而发挥其保护作用。
Objective To investigate the effects of α-melanocyte stimulating hormone (α-MSH) on serum cytokines levels in acute respiratory distress syndrome rats after acute hemorrhagic shock and acute lung injury induced by pulmonary endotoxin. Methods Thirty male Sprague Dawley rats were randomly divided into three groups: control group (A), acute respiratory distress syndrome group (B) and administration group (C ~ F) -1). The changes of serum cytokines (TNF-α, IL-1, IL-6, IL-10) in each group were observed. Results There was no significant difference in the level of serum cytokines (P> 0.05) between the rats in the physiological control group (Group A) at each time point. The severity of acute respiratory distress syndrome The levels of TNF-α, IL-1, IL-6 and IL-10 were significantly increased (P <0.01), and the balance of pro-inflammatory cytokines was unbalanced. (P <0.01), and increased the concentration of IL-6 and IL-10 (P <0.01). The effect of multi-dose group (F group) was the most obvious, while the level of endotoxin There were no significant differences in the levels of cytokines in the drug-treated group (group E) at 1 h after injury compared with those in group B at each time point (P> 0.05). Conclusion α-MSH can reduce serum levels of proinflammatory cytokines in acute respiratory distress syndrome and promote the production of anti-inflammatory cytokines. α-MSH may exert its protective effect by regulating the balance of pro-inflammatory / anti-inflammatory cytokines.