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目的:对行胃镜检出的慢性胃炎患儿行H.pylori检查,分析其检查阳性和阴性患儿的HLA-DQB1等位基因的遗传多态性。方法:病例对照法研究H.pylori阳性和阴性患儿,采用PCR-SSO杂交方法确定HLA-DQB1等位基因型别,病例对照为健康儿童。结果:胃镜检查242例,有上消化道疾病患儿222例,占91.74%。对86例慢性胃炎者行H.pylori检查,阳性57例,占66.28%;阳性患儿DQB1*0602等位基因阳性率低于阴性患儿(χ2=6.97,P<0.01),阳性健康儿DQB1*03032阳性率低于阴性健康儿(χ2=6.97,P<0.01)。结论:胃镜检查儿童上消化道疾病以慢性胃炎为主,H.pylori感染率超过2/3。DQB1*03032和DQB1*0602可能是抵抗H.pylori感染和相关性胃炎的遗传保护因素。
OBJECTIVE: To detect H.pylori in children with chronic gastritis detected by gastroscopy and analyze the genetic polymorphism of HLA-DQB1 alleles in children with positive or negative gastroscopy. Methods: Case-control study of H.pylori positive and negative children, using PCR-SSO hybridization method to determine the HLA-DQB1 allele type, the case control for healthy children. Results: Gastroscopy in 242 cases, 222 cases of upper gastrointestinal diseases in children, accounting for 91.74%. The positive rate of DQB1 * 0602 allele in positive children was lower than that in negative children (χ2 = 6.97, P <0.01), positive healthy children DQB1 * 03032 positive rate was lower than negative healthy children (χ2 = 6.97, P <0.01). Conclusion: Gastroscopy is mainly chronic gastritis in children with upper gastrointestinal diseases, and the infection rate of H.pylori is over 2/3. DQB1 * 03032 and DQB1 * 0602 may be genetic protection against H.pylori infection and related gastritis.