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作者在调研D21Sll和D18S51核酸多态性短串重复序列（STR）标记的基础上，应用定量荧光聚合酶链反应从羊水标本中快速检测18和对三体。该技术用两对引物分别扩增D21511标记和D18551标记。DZISll和D18551的正向引物在5’末端被标记上5一核基荧光素（FAM）。PCRS的总反应体积为50P
On the basis of investigating the short sequence repeat (STR) markers of D21S11 and D18S51, the authors used rapid fluorescent quantitative polymerase chain reaction to detect 18 and trisomy from amniotic fluid samples. This technique uses two pairs of primers to amplify the D21511 marker and the D18551 marker, respectively. The forward primer of DZIS11 and D18551 was labeled with 5-nuclear fluorescein (FAM) at the 5 ’end. The total PCR reaction volume is 50P