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目的 研究促甲状腺激素释放激素 (thyrotrophin- releasing hormone,TRH)及其受体 (TRH recep-tor,TRH- R)在大鼠睾丸组织中的表达规律和在生殖发育调节中的作用。 方法 以大鼠睾丸组织总 RNA为模板 ,依据大鼠下丘脑中的前 TRH和垂体中的 TRH- R c DNA设计引物 ,进行反转录聚合酶链式反应 (reverse tran-scription- polymerase chain reaction,RT- PCR) ,对特异性片段进行序列测定。 结果 从大鼠睾丸组织中获得了前 TRH和 TRH- R的 c DNA片段 ,其核苷酸序列与大鼠下丘脑中的前 TRH和垂体中的 TRH- R c DNA序列完全一致。 结论 首次用 RT- PCR的方法从大鼠睾丸组织中获得了前 TRH和 TRH- R的 c DNA克隆。
Objective To investigate the expression of thyrotrophin-releasing hormone (TRH) and its receptor (TRH recep-tor, TRH-R) in rat testes and its role in the regulation of reproductive development. Methods Using total RNA from rat testis as a template, primers were designed according to TRH in hypothalamus and TRH-R c in pituitary. Reverse tran-scription-polymerase chain reaction , RT-PCR), the specific fragment was sequenced. Results The c DNA fragments of pre-TRH and TRH-R were obtained from the rat testis. The nucleotide sequence of the cDNA was exactly the same as the TRH-Rc DNA sequence in the hypothalamus and TRH in the rat hypothalamus. Conclusions For the first time, c DNA clones of TRH and TRH-R were obtained from rat testis by RT-PCR.