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目的:观察环氧橙皮油素对溃疡性结肠炎小鼠治疗作用。方法:BALB/C小鼠分为6组,分别为环氧橙皮油素低、中、高剂量组(80,160,320μmol·L-1),正常组,柳氮磺砒啶肠溶片组(520 mg·kg-1)和葡聚糖硫酸钠(DSS)模型组。除模型组外均按0.01 m L·g-1体重剂量ig给药,正常组给予等体积的生理盐水。除正常组外各给药组在给药同时自由饮用4%DSS水溶液,连续造模10 d。观察各组药物对BALB/C小鼠疾病活动指数的影响;采用结肠损伤评分法,观察各组药物对病变肠组织的影响;ELISA法测结肠组织细胞因子肿瘤坏死因子-α(TNF-α),白细胞介素-10(IL-10)含量;Western blot法测定环氧橙皮油素对受损结肠平滑肌肌球蛋白轻链激酶(MLCK)表达水平影响。结果:与模型组比较,环氧橙皮油素中、高剂量组疾病活动指数明显降低(P<0.05),结肠损伤指数明显降低(P<0.05),环氧橙皮油素各剂量组结肠组织TNF-α含量逐渐降低,其中高剂量组与模型组有显著差异(P<0.05),环氧橙皮油素各剂量组结肠组织IL-10含量逐渐升高,其中中、高剂量组与模型组均有显著差异(P<0.05),环氧橙皮油素各剂量组均能使受损结肠平滑肌细胞肌球蛋白轻链激酶表达量降低,中、高剂量组与模型组有显著性差异(P<0.05)。结论:环氧橙皮油素通过不同作用机制对溃疡性结肠炎起到治疗作用。
Objective: To observe the therapeutic effect of ephedrine on ulcerative colitis mice. Methods: BALB / C mice were divided into 6 groups: ephedrine low, medium and high dose groups (80,160,320μmol·L-1), normal group, sulfasalazine enteric-coated tablets group mg · kg -1) and dextran sodium sulfate (DSS) model group. Except for the model group, the rats were administered ig at the dose of 0.01 m L · g-1, and the normal rats were given equal volume of normal saline. In addition to the normal group, each administration group was drank freely 4% DSS aqueous solution while administering for 10 days. The effect of each drug on the index of disease activity in BALB / C mice was observed. The colonic damage score method was used to observe the effect of each drug on the diseased intestinal tissue. The levels of tumor necrosis factor-α (TNF-α) , Interleukin-10 (IL-10). Western blot was used to determine the effect of ephedrine on the expression of myosin light chain kinase (MLCK) in injured colonic smooth muscle. Results: Compared with the model group, the activity index of ephedrine in high and medium dose groups was significantly lower (P <0.05) and the index of colonic injury was significantly lower (P <0.05) (P <0.05). The content of IL-10 in colon tissue of each dose of ephedrine increased gradually, and the content of TNF-α in the medium and high dose groups was significantly lower than that of the model group Model group were significantly different (P <0.05), ephedrine each dose group can make damaged colonic myositis light chain kinase expression decreased, medium and high dose group and the model group was significant Difference (P <0.05). Conclusion: Ephedra play a therapeutic role in ulcerative colitis through different mechanism of action.