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利用RT_PCR方法从拟南芥 (Arabidopsisthaliana (L .)Heynh .)中克隆了生长素结合蛋白 (auxinbindingprotein 1 )cDNA ,进行了全序列测定。将该基因克隆在pBI1 2 1的 35S启动子和Nos终止子之间 ,得到植物表达载体p35EZ。通过根癌农杆菌 (Agrobacteriumtumefaciens (SmithetTownsend)Conn)介导的方法转化黄瓜 (CucumissativusL .)。对转基因植株进行了PCR和Southern检测。所得到的转基因黄瓜植株单性结实能力增强。
The cDNA of auxin binding protein 1 (IAA) was cloned from Arabidopsisthaliana (L.) Heynh by RT_PCR and sequenced. This gene was cloned between the 35S promoter of pBI12i and the Nos terminator to give the plant expression vector p35EZ. Cucumber (Cucumissativus L.) was transformed by Agrobacterium tumefaciens (SmitheTownsend) Conn mediated method. Transgenic plants were subjected to PCR and Southern blot. The obtained transgenic cucumber plants have enhanced ability of uniparental fruiting.