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目的：探讨造血生长因子不同组合方式对脐血有核细胞的扩增作用以及脐血扩增后Ｔ淋巴细胞和ＨＬＡ－ＡＢ、ＤＲ阳性细胞变化情况。方法：在脐血有核细胞体外培养体系中分别加入不同组合的细胞因子，观察细胞数、ＣＤ３４＋细胞、ＣＦＵ－ＧＭ、Ｔ４（ＣＤ４＋ＣＤ８－ＣＤ３＋）细胞、Ｔ８（ＣＤ４－ＣＤ８＋ＣＤ３＋）细胞以及ＨＬＡ－ＡＢ、ＤＲ阳性细胞的变化。结果：脐血有核细胞在体外液体培养体系中，经重组人干细胞因子（ｒｈＳＣＦ）、白细胞介素（ＩＬ）－３、ＩＬ－６、粒细胞集落刺激因子（Ｇ－ＣＳＦ）、粒－巨噬细胞集落刺激因子（ＧＭ－ＣＳＦ）和红细胞生成素（Ｅｐｏ）的作用下扩增效果最佳。结论：脐血有核细胞在各种造血生长因子的协同作用下可以在体外扩增。脐血扩增后Ｔ４、Ｔ８细胞减少，可能会降低脐血移植急性ＧＶＨＤ发生，但ＨＬＡ－ＡＢ和ＨＬＡ－ＤＲ阳性细胞数急剧上升，增加了免疫排斥的可能性。
Objective: To investigate the effect of different combinations of hematopoietic growth factors on the proliferation of cord blood nucleated cells and the changes of T lymphocytes, HLA-AB and DR positive cells after cord blood expansion. Methods: Different combinations of cytokines were added into the culture system of cord blood nucleated cells in vitro. The number of cells, CD34 + cells, CFU-GM, T4 (CD4 + CD8 + CD3 +) cells and T8 (CD4- CD8 + CD3 + , DR positive cells changes. RESULTS: The umbilical cord blood mononuclear cells were transfected with recombinant human stem cell factor (rhSCF), interleukin (IL) -3, IL-6, granulocyte colony stimulating factor GM-CSF and erythropoietin (Epo) under the action of amplification best. CONCLUSION: Umbilical cord blood nucleated cells can be expanded in vitro under the synergistic effects of various hematopoietic growth factors. The reduction of T4 and T8 cells after umbilical cord blood expansion may reduce the occurrence of acute GVHD in cord blood transplantation, but the number of HLA-AB and HLA-DR positive cells increases sharply, increasing the possibility of immune rejection.