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本文报道了武汉地区新生儿及小婴儿人类轮状病毒(HRV)感染的研究结果。采用微型简易凝胶电泳法(PAGE)与ELISA法对250份新鲜粪样进行轮状病毒检测,两者均阳性者123例,占49.2%;表明新生儿及小婴儿腹泻的半数是轮状病毒感染。在HRV-PAGE-RNA阳性病例中,收回58份脐血和小婴儿静脉血,其HRV-CF-Ab滴度≥1:4～1:128者48例(82.75%),与粪便配对进行平行试验,其HRV-PAGE-RNA的阳性率达67.3%,新生儿大便HRV阳性率不比小婴儿阳性率低,提示新生儿及小婴儿抗HRV抗体不能保护其免受轮状病毒感染。
This article reports the results of studies on human rotavirus (HRV) infection in newborns and infants in Wuhan. Rotavirus was detected in 250 fresh samples by mini gel electrophoresis (PAGE) and ELISA. 123 cases were positive, accounting for 49.2% of all cases; it showed that half of newborns and infants with diarrhea were rotavirus infection. In HRV-PAGE-RNA positive cases, 58 cases of umbilical cord blood and small infant venous blood were withdrawn and 48 cases (82.75%) with HRV-CF-Ab titer ≥1: 4-1: 128 were paired with feces Test, HRV-PAGE-RNA positive rate of 67.3%, neonatal stool HRV positive rate of positive rate of less than the baby, suggesting that neonatal and small infant anti-HRV antibodies can not protect them from rotavirus infection.