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目的 研究 1 5℃低温海水浸泡对失血性休克大鼠血流动力学和一些生化代谢的影响。方法 采用雄性 Wistar大鼠 ,分为 3组 :正常对照组 (n=8) ;失血性休克组 (n=8) ;海水浸泡失血性休克组 (n=8)。测定血流动力学 ,血乳酸 (L A)、乳酸脱氢酶 (L DH )、磷脂酶 A2 (PL A2 ) ,血液和组织 MDA,分离肝线粒体 ,测定线粒体 H+ - ATP酶和琥珀酸脱氢酶 (SDH)。结果 海水浸泡失血性休克大鼠血浆L A、L DH、PL A2 、血浆和组织 MDA均显著高于失血性休克组和对照组 ,而血流动力学、肝细胞、心肌细胞线粒体 H+ - ATP酶和 SDH均显著低于失血性休克组和对照组。结论 1 5℃海水浸泡失血性休克大鼠伤情明显加重 ,血流动力学减弱 ,缺血再灌流损伤加重 ,线粒体酶及能量合成和分解均下降 ;代谢性酸中毒加重 ,磷脂酶活性升高 ,膜通透性升高
Objective To investigate the effects of seawater immersion at 15 ℃ on hemodynamics and some biochemical metabolism in hemorrhagic shock rats. Methods Male Wistar rats were divided into three groups: normal control group (n = 8), hemorrhagic shock group (n = 8) and seawater immersion hemorrhagic shock group (n = 8). Hemodynamics, lactate dehydrogenase (L DH), phospholipase A2 (PL A2), MDA in blood and tissues were measured and the mitochondria were separated from mitochondria, mitochondrial H + - ATPase and succinate dehydrogenase (SDH). Results The plasma levels of LA, L DH, PL A2, plasma and tissue MDA in seawater soaked in hemorrhagic shock rats were significantly higher than those in hemorrhagic shock and control groups, while hemodynamics, hepatocytes, mitochondrial H + - ATPase and SDH were significantly lower than hemorrhagic shock group and control group. CONCLUSIONS: Injuries in hemorrhagic shock rats exposed to seawater at 5 ℃ were significantly worsened, hemodynamics weakened, increased ischemia-reperfusion injury, decreased mitochondrial enzymes and energy synthesis and degradation, increased metabolic acidosis and increased phospholipase activity , Membrane permeability increased