论文部分内容阅读
组织蛋白酶L(Cathepsin L,CatL)属于木瓜蛋白酶,CatL由一个信号肽、一个前肽和一个酶的催化反应区组成。在一系列信号肽的作用下,CatL定位到高尔基体,并在此被高甘露糖碳水化合物转化化为CatL酶原,与两个6-磷酸甘露糖受体中的一个结合形成复合物,然后被转运至前溶酶体。此处的酸性环境可使酶和受体复合物分离,并促进酶的激活形成成熟的CatL,经过一系列反应形成小分子片段,才能完全活化,经过一系的反应后,被释放到胞质或组织间隙,可降解细胞成分或细胞间质基质成分,参与肿瘤的浸润与转移、关节炎、骨质疏松等慢性炎症性疾病的发生发展过程。近年来的研究发现,CatL在心血管疾病组织中有表达,提示组织蛋白酶L在心血管疾病中也起着非常重要的作用。
Cathepsin L (CatL) belongs to papain, and CatL consists of a signal peptide, a propeptide and an enzymatic catalytic reaction zone. CatL is localized to the Golgi apparatus under the action of a series of signal peptides, where it is converted to CatL zymogen by a high-mannose carbohydrate and binds to one of the two 6-mannosephosphate receptors to form a complex, which is then Is transported to the pre-lysosome. The acidic environment here separates the enzyme from the receptor complex and promotes the activation of the enzyme to form mature CatL. After a series of reactions to form small molecule fragments, it can be fully activated and released to the cytoplasm after a series of reactions Or interstitial tissue, cell components can be degraded or stromal matrix components, involved in tumor invasion and metastasis, arthritis, osteoporosis and other chronic inflammatory diseases in the development process. Recent studies have found that CatL expression in cardiovascular disease tissue, suggesting that cathepsin L also plays a very important role in cardiovascular disease.