论文部分内容阅读
目的建立樱桃中7种植物生长调节剂(氯吡脲、赤霉素、噻苯隆、多效唑、4-氯苯氧乙酸、2,4-二氯苯氧乙酸和烯效唑)残留的Qu ECh ERS-超高效液相色谱-串联质谱技术同时测定方法。方法样品匀浆后经1%乙酸乙腈提取,分散固相萃取净化,以乙腈和0.01%氨水溶液为流动相进行梯度洗脱,ACQUITY BEH C18色谱柱分离,采用电喷雾多反应监测模式,正、负离子切换,外标法定量。结果 7种植物生长调节剂线性相关系数均>0.99,方法检出限在0.005~1.0μg/kg之间,定量限在0.015~3.0μg/kg之间,7种植物生长调节剂的平均回收率在74.0~101%之间,相对标准偏差小于5%。结论该方法简便、准确、灵敏、重现性好,可应用于樱桃样品的检测。
OBJECTIVE To establish the Qu ECh of seven plant growth regulators (clopidol, gibberellin, thidiazuron, paclobutrazol, 4-chlorophenoxyacetic acid, 2,4-dichlorophenoxyacetic acid and uniconazole) in cherry ERS-UHPLC-tandem mass spectrometry. Methods The samples were homogenized, extracted with 1% acetic acid and purified by dispersive solid-phase extraction. The mobile phases were eluted with acetonitrile and 0.01% aqueous ammonia. The mobile phases were separated by ACQUITY BEH C18 column. Negative ion switching, external standard method. Results The linear correlation coefficients of seven plant growth regulators were both> 0.99. The detection limits were between 0.005-1.0 μg / kg and the limits of quantification were between 0.015-3.0 μg / kg. The average recoveries of seven plant growth regulators Between 74.0 and 101%, the relative standard deviation was less than 5%. Conclusion The method is simple, accurate, sensitive and reproducible. It can be applied to the detection of cherry samples.