目的 观察ⅢA型前列腺炎患者前列腺液(EPS)中锌离子、前列腺特异性抗原(PSA)浓度变化,并探讨其临床意义. 方法 招募ⅢA型前列腺炎患者71例(观察组)及健康查体者44例(对照组). 采用化学比色法、ELISA法分别测定其EPS中锌离子、PSA浓度. 以湿玻片法在显微镜下完成EPS中WBC等级,用pH试纸测定EPS中pH值. 锌离子、PSA浓度及其与慢性前列腺炎症状指数( NIH-CPSI)评分的关系采用Pearson线性相关分析;锌离子、PSA浓度与pH值、WBC等级的关系采用Spearman秩相关分析. 结果 观察组与对照组EPS中锌离子浓度分别为(155.93 ±68.65)、(206.54 ±65.18)μg/mL,PSA浓度分别为(2.27 ±2.03) ×105、(3.78 ±2.84) ×105 ng/mL,两组比较,P均<0.05. 观察组WBC等级偶见0例、1+为32例、2+为21例、3+为17例、4+为1例,对照组均为偶见. 观察组与对照组EPS中pH值分别为6.85 ±0.52、6.58 ±0.42,两组比较,P<0.05. EPS中锌离子浓度与PSA浓度呈正相关(r=0.330,P<0.001). 锌离子浓度与NIH-CPSI评分中的疼痛评分、生活质量影响评分及NIH-CPSI总评分均呈负相关(r分别为-0.278、-0.224、-0.248,P均0.05). PSA浓度与NIH-CPSI评分中的疼痛评分、生活质量影响评分及NIH-CPSI总评分均呈负相关(r分别为-0.255、-0.217、-0.250,P均0.05). 锌离子浓度、PSA浓度与pH值均呈负相关(r分别为-0.429、-0.333,P均<0.001). PSA浓度与WBC等级呈负相关( r=-0 .306 , P0.05).结论 ⅢA型前列腺炎患者EPS中锌离子和PSA浓度显著下降,检测EPS中锌离子、PSA浓度有助于ⅢA型前列腺炎的诊断及病情评估.“,”Objective To observe the changes in concentrations of zinc and prostate-specific antigen ( PSA) in the expressed prostatic secretion ( EPS) of patients with ⅢA prostatitis and to investigate the clinical significance .Methods Seventy-one patients with ⅢA prostatitis ( observation group ) and 44 healthy controls ( control group ) were recruited in our study.The concentrations of zinc and PSA in EPS were determined by colorimetric analysis and enzyme -linked immunosor-bent assay (ELISA), respectively.The wet mount was examined promptly with a high-power microscope for WBC in EPS . The pH value of EPS was acquired by pH test strips .Pearson linear correlation analysis was used to determine the correla-tions between zinc concentration , PSA concentration and CPSI scores .The relationships between zinc concentration and pH value, amount of WBC were analyzed by Spearman rank correlation analysis as well as the relationships between PSA con -centration and pH value , amount of WBC .Results The zinc concentrations of the observation group and control group were respectively (155.93 ±68.65) μg/mL and (206.54 ±65.18) μg/mL, and the PSA concentrations were respectively (2.27 ±2.03) ×105 ng/mL and (3.78 ±2.84) ×105ng/mL (all P<0.05).In the observation group, O case of WBC was occasionally found , 32 cases of 1+, 21 cases of 2+, 17 cases of 3+and 1 case of 4+, and WBC of the controlAbstract:Objective To observe the changes in concentrations of zinc and prostate-specific antigen ( PSA) in the expressed prostatic secretion ( EPS) of patients with ⅢA prostatitis and to investigate the clinical significance .Methods Seventy-one patients with IIIA prostatitis ( observation group ) and 44 healthy controls ( control group ) were recruited in our study.The concentrations of zinc and PSA in EPS were determined by colorimetric analysis and enzyme -linked immunosor-bent assay (ELISA), respectively.The wet mount was examined promptly with a high-power microscope for WBC in EPS . The pH value of EPS was acquired by pH test strips .Pearson linear correlation analysis was used to determine the correla-tions between zinc concentration , PSA concentration and CPSI scores .The relationships between zinc concentration and pH value, amount of WBC were analyzed by Spearman rank correlation analysis as well as the relationships between PSA con -centration and pH value , amount of WBC .Results The zinc concentrations of the observation group and control group were respectively (155.93 ±68.65) μg/mL and (206.54 ±65.18) μg/mL, and the PSA concentrations were respectively (2.27 ±2.03) ×105 ng/mL and (3.78 ±2.84) ×105ng/mL (all P<0.05).In the observation group, O case of WBC was occasionally found , 32 cases of 1+, 21 cases of 2+, 17 cases of 3+and 1 case of 4+, and WBC of the controlgroup was all occasional .The pH values of the observation group and control group were respectively 6.85 ±0.52 and 6.58 ±0.42 (P<0.05).The concentration of zinc was positively correlated with that of PSA (r=0.330, P<0.001).There were negative correlations between zinc concentration and NIH-CPSI scores including pain scores , quality of life scores and total scores (r=-0.278,-0.224 and -0.248, respectively;all P0.05).Meanwhile, there were negative correlations between PSA concentra-tion and NIH-CPSI scores including pain scores , quality of life scores and total scores ( r =-0.255, -0.217 and-0.250, respectively;all P0.05).The pH value was negatively correlated with concentrations of zinc and PSA (r=-0.429 and -0.333, respec-tively;all P<0.001).The PSA concentration was negatively correlated with the amount of WBC (r=-0.306, P0.05).Conclusions The concentrations of zinc and PSA in EPS decreased significantly in patients with ⅢA prostatitis.The detections of concentra-tions of zinc and PSA contributed to the diagnosis and disease evaluation of ⅢA prostatitis.