目的验证脂质体法将wtp53有效转染SHG44细胞。方法G418筛选,DNA分子班点杂交。结果用脂质体法将wtp53质粒转染SHG44细胞,G418筛选,24d形成新生细胞克隆,呈集落状;以wtp53cDNA为探针进行斑点杂交,放射自显影,wtp53pLXSN/SHG44细胞为阳性杂交信号,而亲代SHG44细胞及转染pLXSN的SHG44细胞为阴性信号,说明wtp53基因已成功地转导入SHG44细胞中。结论斑点分子杂交显示,基因转染成功,初步证实脂质体介导法能有效地将外源性p53基因转导入SHG44细胞。 Objective To verify the efficiency of liposome transfection of SH-p53 into SHG44 cells. Methods G418 screening, DNA molecular class spot hybridization. RESULTS: The p53 plasmid was transfected into SHG44 cells by liposome method and screened with G418. The new cell clone was formed on 24 d after colony formation. Dot-blot hybridization with wt p53 cDNA as the probe, autoradiography, and wt p53pLXSN/SHG44 cells were performed. As a positive hybridization signal, the parental SHG44 cells and SHG44 cells transfected with pLXSN were negative signals, indicating that the wt p53 gene has been successfully transduced into SHG44 cells. [Conclusion] The dot blotting showed that the gene transfection was successful, and it was initially confirmed that the liposome-mediated method can effectively transduce the exogenous p53 gene into SHG44 cells.