论文部分内容阅读
[目的]研究福州市人群乙肝流行规律,探讨沿海地区HBV高发的原因,为制定防控策略提供科学依据。[方法]按整群抽样法,以家庭为单位随机抽样,用ELISA方法检测乙肝血清标志物,用荧光定量PCR检测HBV-DNA含量,用型特异性PCR进行乙肝病毒基因分型。[结果]福州市检测结果,人群HBsAg、HBsAb、HBeAg、HBeAb、HBcAb阳性率分别是13.3%、53.3%、2.3%、17.0%、56.8%;沿海2558份标本阳性率分别是16.8%、45.6%、4.9%、15.8%、53.9%;内陆1635份标本阳性率分别是9.7%、47.2%、2.2%、15.0%、51.5%;沿海HBsAg、HBeAg阳性率高于内陆(P<0.05)。随机抽取323份DNA阳性的样品进行基因分型,B型占49.2%、C型占43.4%、B+C型占7.4%,沿海B、C型比重相近,内陆以B型为主。[结论]福州市人群中HBsAg携带率为13.3%,高于全国平均水平。沿海HBsAg携带率高达16.8%,为福州市乙型肝炎高发人群,应加强乙肝的预防与控制。
[Objective] To study the prevalence of hepatitis B in population of Fuzhou and to explore the causes of high incidence of HBV in coastal areas, and to provide scientific basis for making prevention and control strategies. [Methods] According to cluster sampling method, samples were randomly collected from families. Serum HBV markers were detected by ELISA. HBV-DNA content was detected by fluorescence quantitative PCR and genotyped by genotype-specific PCR. [Results] The positive rates of HBsAg, HBsAb, HBeAg, HBeAb and HBcAb in Fuzhou were 13.3%, 53.3%, 2.3%, 17.0% and 56.8% respectively. The positive rates of 2558 samples in coastal area were 16.8% and 45.6% , 4.9%, 15.8% and 53.9% respectively. The positive rates of HBsAg and HBeAg in the inland were 9.7%, 47.2%, 2.2%, 15.0% and 51.5% respectively. A total of 323 DNA positive samples were randomly selected for genotyping. Type B accounted for 49.2%, type C accounted for 43.4% and type B + C accounted for 7.4%. Coastal B and C types had similar specific gravities, while inland type B mainly. [Conclusion] The population carrying rate of HBsAg in Fuzhou is 13.3%, higher than the national average. Coastal HBsAg carrier rate as high as 16.8%, for the high incidence of hepatitis B in Fuzhou, hepatitis B should be strengthened prevention and control.