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目的探讨三色流式细胞术在儿童急性白血病AL免疫分型中的应用价值,了解儿童AL抗原表达规律和免疫亚型的分布情况。方法采用CD45/侧散射(SSC)双参数散点图设门方法进行三色流式细胞术细胞表面及浆内分化抗原的分析。结果120例儿童白血病免疫分型可分为4类:未分化型占0·8%,急性髓细胞性白血病(AML)占35·0%,急性淋巴细胞白血病(ALL)占57·5%,混合型急性白血病占6·7%。69例儿童ALL中,B淋巴细胞白血病(B-ALL)占75·3%,T淋巴细胞白血病(T-ALL)占24·7%。AML伴淋巴系抗原表达为28·8%,主要表达CD5、CD7及CDl9(均为9·6%)。B-ALL伴髓系抗原CDl3、CD33表达分别为40·4%和5·8%,T-ALL伴髓系抗原CD13、CD33表达分别为35·3%和5·9%。结论应用流式细胞术几乎能对所有儿童急性白血病进行准确分型,对儿童白血病患者的治疗方案选择及预后判断等均有重要价值。
Objective To investigate the value of three-color flow cytometry in the classification of AL in acute leukemia in children and to find out the distribution of AL antigen and immunologic subtype in children. Methods Three-color flow cytometry (FCM) was used to analyze the cell surface and the differentiated antigen in plasma by CD45 / SSC double-parameter scatter plot. Results 120 cases of childhood leukemia immunophenotypes can be divided into four categories: undifferentiated type accounted for 0.8%, acute myeloid leukemia (AML) accounted for 35.0%, acute lymphoblastic leukemia (ALL) accounted for 57.5% Mixed acute leukemia accounted for 6.7%. Among 69 children with ALL, B-ALL accounted for 75.3% and T-ALL accounted for 24.7%. AML with lymphoid antigen expression was 28.8%, mainly expressed CD5, CD7 and CD19 (both 9.6%). The expressions of CD33 and CD33 of B-ALL with myeloid antigens were 40.4% and 5.8%, respectively. The expressions of CD13 and CD33 of T-ALL with myeloid antigens were 35.3% and 5.9%, respectively. Conclusion Flow cytometry can be used to classify acute leukemia in almost all children, and has important value in treatment options and prognosis of childhood leukemia patients.