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目的观察冠状动脉内注射法移植自体骨骼肌成肌细胞到无再灌注急性心肌梗死区后的生长分化特点。方法结扎犬冠状动脉前降支中段,建立急性心肌梗死模型;杂种成年犬10只,分为对照组和经冠状动脉注射移植组,各5只。经梗死相关冠状动脉内注射自体骨骼肌成肌细胞悬液10 ml(1.0~1.4×108个)或等量生理盐水;4周后通过HE染色、PTH染色、骨骼肌特异性慢肌球蛋白抗体免疫组织化学染色和透射电镜评价移植细胞病理转归。结果经冠状动脉内注射移植自体骨骼肌成肌细胞4周后,透射电镜及HE染色下可在梗死区内找到新生幼稚肌源性细胞存在,PTH染色证实有新生的横纹肌组织形成,骨骼肌特异性慢肌球蛋白抗体免疫组织化学染色发现有骨骼肌源性的成熟肌组织存在且新生肌组织排列较分散。结论通过经梗死相关冠状动脉注射将自体骨骼肌成肌细胞移植到急性心肌梗死区后能形成成熟的肌组织。
OBJECTIVE: To observe the growth and differentiation of autologous skeletal myoblasts after intracoronary injection into the area of acute myocardial infarction without reperfusion. Methods Acute myocardial infarction (AMI) model was established by ligation of the middle part of the anterior descending coronary artery in dogs. Ten hybrid adult dogs were divided into control group and transplanted coronary artery. 10 ml (1.0-1.4 × 108) or normal saline were injected into the infarct-related coronary artery via the coronary artery. After 4 weeks, HE staining, PTH staining, skeletal muscle-specific myosin antibody Immunocytochemical staining and transmission electron microscopy were used to evaluate the pathological changes of the transplanted cells. Results Autologous skeletal muscle myoblasts were transplanted intracoronary injection for 4 weeks. Transmission electron microscopy and HE staining showed that neonatal naive myogenic cells were found in the infarct area. PTH staining confirmed the formation of new striated muscle and skeletal muscle Immunohistochemical staining of sex-specific slow-myosin antibodies revealed skeletal muscle-derived mature muscle tissue and newborn muscle tissue was more dispersed. Conclusion Autologous skeletal muscle myoblasts transplanted into acute myocardial infarction area through infarction-related coronary injection can form mature muscle tissue.