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目的了解本地区女性人乳头瘤病毒(HPV)感染状况,为预防HPV感染和宫颈疾病防治提供理论依据。方法应用导流杂交基因芯片技术(可以同时检查21种基因型别),对21例宫颈癌、59例CIN、38例宫颈炎症1、006例宫颈正常的共1 124例宫颈细胞标本进行HPV检测及基因分型,分析其感染情况及型别分布。采用卡方检验,对HPV阳性和阴性在不同程度宫颈病变的构成比进行分析。结果本组资料中总感染率为28.4%(319/1 124),存在多重感染。共查出20种基因型,HPV43型未查出。HPV的主要基因型别为HPV16(19.8%)、HPV58(18.3%)、HPV52(16.1%)、HPV18(6.3%)。HPV阳性与阴性组之间在宫颈正常、宫颈炎症、宫颈CIN和宫颈癌的发病总体构成比差异有统计学意义(χ2=150.4,P<0.005)。结论 HPV16、HPV58、HPV52、HPV18为该地区HPV感染的主要型别,感染高危型HPV是引起宫颈CIN及宫颈癌的必要和关键因素。HPV检测能及早发现宫颈疾病,在有效防治宫颈癌前病变,降低宫颈癌症的发病率中有重要的意义。
Objective To understand the status of human papillomavirus (HPV) infection in this area and provide a theoretical basis for the prevention of HPV infection and prevention and treatment of cervical diseases. Methods A total of 1 124 cases of cervical cancer from 21 cases of cervical cancer, 59 cases of CIN, 38 cases of cervix inflammation and 1,006 cases of normal cervix were tested for HPV by flow-through hybridization and gene chip technique (21 genotypes could be examined simultaneously) And genotyping, analysis of their infection and type distribution. Using chi-square test, HPV positive and negative in varying degrees of cervical lesions constituent ratio analysis. Results The total infection rate in this group was 28.4% (319/1 124), with multiple infections. A total of 20 genotypes were detected, while HPV43 type was not detected. The major genotypes of HPV were HPV16 (19.8%), HPV58 (18.3%), HPV52 (16.1%) and HPV18 (6.3%). There were significant differences in the overall constitutional ratio between HPV positive and negative groups in cervical normal, cervical inflammation, cervical CIN and cervical cancer (χ2 = 150.4, P <0.005). Conclusion HPV16, HPV58, HPV52 and HPV18 are the main types of HPV infection in this area. Infection with high-risk HPV is the necessary and key factor to cause cervical CIN and cervical cancer. HPV testing can detect cervical disease as early as possible, and is of great significance in effectively preventing and treating cervical precancerous lesions and reducing the incidence of cervical cancer.