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刺蛾科昆虫是柞园栎树林中的重要害虫类群。克隆了栎树林中主要刺蛾科昆虫的线粒体细胞色素C氧化酶亚基Ⅰ基因(mtDNA COⅠ,Gen Bank登录号:KP727647~KP727664),以此作为供试刺蛾科昆虫的DNA条形编码基因分析其碱基组成特点和遗传进化规律,探讨将其应用于栎树林中刺蛾科害虫的分类鉴定和系统进化关系研究的可行性。刺蛾科昆虫各物种间的mtDNA COⅠ基因碱基组成差异不明显,碱基T、C、A、G的平均含量分别为38.0%、16.2%、30.7%和15.1%,AT含量为68.7%,显著高于GC含量(31.3%),表现出明显的AT使用趋向性;碱基明显倾向于使用T(AT偏倚度为-0.106 26),具有较弱的C偏好性(GC偏倚度为-0.035 14),不同物种间该基因碱基T使用偏好程度差异较大。刺蛾科昆虫mtDNA COⅠ基因碱基变异率为26.64%,碱基颠换明显高于转换(R值为0.75)。刺蛾科昆虫各物种mtDNA COⅠ基因的平均进化率为11.5%,进化率最大的发生在锯纹岐刺蛾Austrapoda seres与中国扁刺蛾Thosea sinensis间,进化率最小的发生在黄刺蛾Monema flavescens与梨娜刺蛾Narosoideus flavidorsalis之间。在刺蛾科昆虫mtDNA COⅠ基因的系统进化树中,中国绿刺蛾Parasa sinica和中国扁刺蛾Thosea sinensis聚在一起形成一个单系,其他物种聚在一起形成另外一个单系。以上结果表明,mtDNA COⅠ基因可作为DNA条形编码用于栎树林刺蛾科害虫的分类鉴定和系统进化研究。
The moth insect is an important pest taxa in oak orchard. The mitochondrial cytochrome C oxidase subunit Ⅰ gene (mtDNA COⅠ, GenBank accession number: KP727647 ~ KP727664) was cloned from the major larvae of the oak forest, and was used as the DNA barcode gene The characteristics of base composition and genetic evolution were analyzed, and the feasibility of its application in taxonomic identification and phylogenetic relationship of the litura pests in oak forest was discussed. There was no significant difference in the base composition of the mtDNA COⅠ gene among the species of the Lycoptera. The average contents of T, C, A and G were 38.0%, 16.2%, 30.7% and 15.1%, respectively. The AT content was 68.7% Significantly higher than the GC content (31.3%), showing a significant tendency toward AT use; bases tended to use T (AT bias -0.106 26) with weaker C bias (GC bias -0.035 14). The degree of preference of base T in different species varies greatly among different species. The mutation rate of mtDNA COⅠ gene was 26.64%. The transversion of base was obviously higher than that of conversion (R value 0.75). The average evolution rate of mtDNA COⅠ gene in each species was 11.5%. The most evolutionary rate occurred between Austrapoda seres and Peoplea sinensis, among which the lowest evolution rate occurred in Monema flavescens With pear Narinaoideus flavidorsalis. In the phylogenetic tree of the mtDNA COⅠ gene from the moth, the Chinese green moth Parasa sinica and the Chinese flat-moth Plastron from the sinensis converge to form a single lineage. Other species come together to form another single lineage. The above results showed that the mtDNA COⅠ gene could be used as a DNA barcode for classification and phylogenetic analysis of the insect pest of the oak forest.