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结合形态学和分子生物学方法,对采自新疆额尔齐斯河河鲈肠道的三枝钩绦虫幼虫进行了鉴定。通过对采集的绦虫幼虫的形态特征进行观察,发现虫体头节有2条浅的吸沟,前端有1个不明显的吸盘,盘上有4个三叉形的小钩,初步鉴定为三枝钩绦虫属的种类。通过扩增18SrDNA序列,发现采集的三枝钩绦虫幼虫与GenBank上公布的结节三枝钩绦虫的18SrDNA序列的同源性为99.5%,该序列已在GenBank中提交,获得的登录号为KM272003.1。同时,利用GenBank中三枝钩科的8个属的代表虫种的18SrDNA序列构建系统发育树,发现本研究中采集的绦虫与新西兰和瑞士报道的结节三枝钩绦虫聚在同一分支。上述结果表明,新疆额尔齐斯河的河鲈肠道寄生的三枝钩绦虫幼虫为结节三枝钩绦虫。
In combination with morphological and molecular methods, the larvae of three hookworm larvae collected from the intestinal tract of the river bass in the Irtysh, Xinjiang Province were identified. By observing the morphological characteristics of collected tapeworm larvae, it was found that there were two shallow sucking ditches in the head of the larvae, one inconspicuous sucker at the front end, and four tripartite hooks on the tray, initially identified as three hooks Tapeworm genus. The amplified 18S rDNA sequence showed that the homology of the collected three T. cestodon larvae to the 18S rDNA sequence of the three T. coccacia nodules published in GenBank was 99.5%. The sequence was submitted in GenBank and the accession number was KM272003.1 . At the same time, phylogenetic tree was constructed using 18S rDNA sequences of 8 genera of three genera in GenBank. It was found that the tapeworms collected in this study were clustered together with three Trichomoniasis reported in New Zealand and Switzerland. The above results show that the trichlorfon larvae parasitized in the intestinal tract of the bass in Xinjiang’s Irtysh River are nodular triacanthons.