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通过开环聚合法合成了聚乙二醇-聚己内酯嵌段共聚物(PEG-b-PCL),以PEG-b-PCL为囊壁、以戊唑醇为囊芯,采用自组装的方法制备了载药胶束。分别利用动态光散射仪(DLS)和透射电镜(TEM)对其粒径进行了表征,通过紫外分光光度计和高效液相色谱仪测定了其载药量、包覆率和体外释药性能,用滤纸片法测试了载药胶束对木霉Trichoderma viride的抑菌性能。结果表明:PEG-b-PCL载戊唑醇胶束平均粒径为250 nm,均匀度良好,载药量为26.4%,包覆率为18.0%,体外释放192 h的释药率为83.35%,其对木霉的抑菌效果明显高于同浓度下的戊唑醇乙酸乙酯溶液。
Polyethylene glycol-polycaprolactone block copolymer (PEG-b-PCL) was synthesized by ring-opening polymerization. PEG-b-PCL was used as the capsule wall and tebuconazole as the capsule core. Methods Preparation of drug-loaded micelles. The particle size was characterized by dynamic light scattering (DLS) and transmission electron microscopy (TEM) respectively. The drug loading, coating rate and drug release in vitro were determined by ultraviolet spectrophotometer and high performance liquid chromatography. Antibacterial activity of drug-loaded micelles against Trichoderma viride was tested by filter paper method. The results showed that the average particle size of PEG-b-PCL loaded tebuconazole micelles was 250 nm with good uniformity, loading rate was 26.4%, coverage rate was 18.0%, release rate was 83.35% , Its inhibitory effect on Trichoderma significantly higher than the same concentration of tebuconazole ethyl acetate solution.