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[Objective]To determine the content of aesculin in the leaves of Fraxinus chinensis. [Methods]The sample was extracted after it had undergone reflux through absolute methanol,and the filler was the bonding of octadecyl silicon and silica gel; the acetonitrile- 0. 1%concentration phosphoric acid solution(8∶92,V /V) was taken as mobile phase,the wavelength measured was 334 nm,the column temperature was 30 ℃,and the flow velocity was 1. 0 mL /min. The content was determined via high performance liquid chromatography. [Results]The average recovery rate was 96. 59%,the RSD was 2. 39%(n = 6),and the average content of aesculin in the leaves of Fraxinus chinensis was0. 155 9 mg /g. [Conclusions]This method is of high stability,accuracy and reproductivity,and thus suitable for determining aesculin in the leaves of Fraxinus chinensis.
[Objective] To determine the content of aesculin in the leaves of Fraxinus chinensis. [Methods] The sample was extracted after it had undergone reflux through absolute methanol, and the filler was the bonding of octadecyl silicon and silica gel; The concentration of phosphoric acid solution (8:92, V / V) was taken as mobile phase, the wavelength measured was 334 nm, the column temperature was 30 ° C and the flow velocity was 1.0 mL / min. The content was determined via high performance liquid chromatography. [Results] The average recovery rate was 96. 59%, the RSD was 2. 39% (n = 6), and the average content of aesculin in the leaves of Fraxinus chinensis was0. 155 9 mg / g. [Conclusions] This method is of high stability, accuracy and reproductivity, and thus suitable for determining aesculin in the leaves of Fraxinus chinensis.