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目的:探讨缺氧抑制因子-1α(HIF-1α)和白细胞介素-6(IL-6)对子痫前期的发病机制及影响胎儿发育的作用。方法:其中足月择期行剖宫产的健康孕妇40例为正常对照组、子痫前期且胎儿发育正常的孕妇40例作为PE组、子痫前期且胎儿生长受限(FGR)的孕妇40例作为PE+FGR组。运用实时定量PCR(realtime-PCR)技术及蛋白印迹法(WB)检测各组孕妇胎盘组织中HIF-1α和IL-6 mRNA和蛋白的表达。结果:HIF-1α和IL-6 mRNA和蛋白的表达量:PE组和PE+FGR组与正常对照组比较,差异有统计学意义(P<0.01)。但PE组和PE+FGR组中HIF-1α和IL-6 mRNA及蛋白的表达比较,差异有统计学意义(P<0.05)。结论:胎盘性HIF-1α和IL-6对于导致子痫前期的发病及胎儿的正常发育具有重要的作用,为子痫前期的诊断及治疗提供了相关的应对策略。
Objective: To investigate the effects of hypoxia-inducible factor-1α (HIF-1α) and interleukin-6 (IL-6) on the pathogenesis of preeclampsia and its effect on fetal development. Methods: Forty pregnant women undergoing full-term elective cesarean delivery were enrolled in this study. Forty pregnant women with preeclampsia and normal fetal development were enrolled as PE group, 40 pregnant women with preeclampsia and fetal growth restriction (FGR) As PE + FGR group. The expression of HIF-1α and IL-6 mRNA and protein in placenta of each group were detected by real-time PCR and Western blotting. Results: The mRNA and protein expressions of HIF-1α and IL-6 in PE group and PE + FGR group were significantly different from those in normal control group (P <0.01). However, the expression of HIF-1α and IL-6 mRNA and protein in PE and PE + FGR groups was significantly different (P <0.05). Conclusion: The placental HIF-1α and IL-6 play an important role in the pathogenesis of preeclampsia and the normal development of the fetus, and provide relevant strategies for the diagnosis and treatment of preeclampsia.