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背景:近年来,干细胞替代治疗为神经再生提供了新的方法,但干细胞移植治疗小儿脑发育不全的相关报道甚少。目的:验证嗅鞘细胞联合神经干细胞移植治疗小儿脑发育不全的有效性。方法:2009-10解放军第163医院收治了1例脑发育不全的患者,给予物理降温、神经营养、维持水电解质平衡、高压氧治疗的同时,以L3～4腰椎间隙为穿刺点,将配置好的神经干细胞约8×107个和嗅鞘细胞约4×107个缓慢注入蛛网膜下腔,每2周1次,2次为1个疗程,共治疗1个疗程。从体温恢复程度、双手精细动作、运动协调性、语言等方面来评价移植治疗效果。结果与结论:与移植前比较,嗅鞘细胞联合神经干细胞移植1个月后患儿体温恢复正常,四肢肌力明显下降,2个月后可以独自端坐,3个月后可以扶梯行走,有简单的语言表达。提示嗅鞘细胞联合神经干细胞移植治疗小儿脑发育不全是有效的,能恢复其中枢神经功能。
Background: In recent years, stem cell replacement therapy has provided a new method for nerve regeneration. However, there are few reports on stem cell transplantation for treatment of cerebral hypoplasia in children. OBJECTIVE: To verify the effectiveness of olfactory ensheathing cells combined with neural stem cell transplantation in the treatment of cerebral hypoplasia in children. Method: 2009-10 People’s Liberation Army 163 Hospital admitted to a patient with brain hypoplasia, given physical cooling, neurotrophic, maintain water and electrolyte balance, hyperbaric oxygen therapy at the same time, L3 ~ 4 lumbar intercostal puncture point, will be configured Of neural stem cells about 8 × 107 and olfactory ensheathing cells about 4 × 107 into the subarachnoid space slowly, once every 2 weeks, 2 times for a course of treatment for a course of treatment. From the degree of body temperature recovery, his hands fine movement, motor coordination, language and other aspects to evaluate the effect of transplantation. RESULTS AND CONCLUSION: Compared with those before transplantation, the body temperature of children with normal olfactory ensheathing cells combined with neural stem cell transplantation returned to normal after one month. The muscle strength of the limbs decreased obviously. After 2 months, they could sit alone and escalator after 3 months. Simple language expression. Tip olfactory ensheathing cells combined with neural stem cell transplantation in children with cerebral hypoplasia is effective and can restore its central nervous system function.