目的对榄香烯原料药化学成分进行系统研究。方法采用氧化铝柱色谱、硅胶柱色谱和制备型HPLC等分离方法从榄香烯原料药中分离化合物,采用NMR等光谱学手段对它们进行结构鉴定。结果共分离得到4个化合物,分别鉴定为β-榄香烯(1)、γ-榄香烯(2)、β-石竹烯(3)和δ-榄香烯(4)。结论不仅对榄香烯原料药的3个主要活性成分进行了结构确证,而且对原料药中的1个主要杂质成分β-石竹烯进行了结构确证,并首次对文献中β-石竹烯碳谱数据中有误的归属进行了纠正。为榄香烯原料药质量控制和临床试验的研究提供了明确的物质基础。 Objective To systematically study the chemical constituents of elemene. Methods The compounds were separated from elemene by HPLC, silica gel column chromatography and preparative HPLC. Their structures were identified by spectroscopic methods such as NMR. Results Four compounds were isolated and identified as β-elemene (1), γ-elemene (2), β-caryophyllene (3) and δ-elemene (4). Conclusion The structure of the three main active components of elemene was confirmed. The structure of β-Caryophyllene, one of the main impurities in the drug substance, was confirmed. For the first time, The ownership of the data was corrected. Elemene APIs for quality control and clinical trials provide a clear material basis for the study.