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目的 观察环境激素壬基苯酚(NP)对豚鼠心室肌细胞L-广型钙电流(I_(Ca-L))的影响.方法 应用酶机械法分离豚鼠单个心室肌细胞;应用膜片钳全细胞方法观察NP对单个心室肌细胞I_(Ca-L)的影响.结果 NP可抑制不同膜电位下的I_(Ca-L),NP(10~(-6) mol·L~(-1)、10~(-5) mol·L~(-1))使I_(Ca-L)峰值电流密度从(-3.2±1.5)pA.pF-1减少到(-1.6±0.8)pA·pF~(-1)(P＜0.01)和(-1.4±0.7)pA·pF~(-1)(P＜0.01);但对I_(Ca-L)激活动力学曲线无明显影响.结论 NP可剂量依赖性抑制豚鼠心室肌细胞I_(Ca-L).“,”Objective To observe the effects of nonylphenol(NP) on L-type Ca~(2+) currents(I_(Ca-L)) in isolated guinea-pig ventricular myocytes.Methods The ventricular myocytes were isolated from guinea-pig hearts.The L-type Ca~(2+) currents in the ventricular myocytes treated with NP were measured with whole cell patch-clamp technique.Results Nonylphenol inhibited lew,at different potentials.Nonylphenol (10~(-6) mol·L~(-1),10~(-5) mol·L~(-1)) reduced the peak amplitude of I_(Ca-L) from-3.2±1.5 pA·pF~(-1) to-1.6±0.8 pA·pF~(-1)(P<0.01) and-1.4±0.7 pA·pF~(-1)(P<0.01),respectively.Nonylphenol did not influence the activation curve of I_(Ca-L) significantly.Conclusion Nonylphenol could in hibit the L-type calcium currents in isolated guinea-pig ventricular myocytes with a concentration-dependent manner.Objective To observe the effects of nonylphenol(NP) on L-type Ca~(2+) currents(I_(Ca-L))in isolated guinea-pig ventricular myocytes.Methods The ventricular myocytes were isolated from guinea-pig hearts.The L-type Ca~(2+) currents in the ventricular myocytes treated with NP were measured with whole cell patch-clamp technique.Results Nonylphenol inhibited lew,at different potentials.Nonylphenol hibit the L-type calcium currents in isolated guinea-pig ventricular myocytes with a concentration-dependent manner.