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目的鉴定抗肿瘤药物20(R)-25-OCH3-PPD在大鼠体内的代谢产物。方法大鼠单次灌胃给予药物20(R)-25-OCH3-PPD(40 mg·kg-1),收集粪便、尿液及血浆。采用LC-MS/MS法,以多反应监测(MRM)和二级全扫描质谱(Full Scan MS2)方式,对大鼠粪便、尿液及血浆中代谢产物进行分析和鉴定。结果在粪中检测到原形药物及8个代谢物,在血浆中检测到原形药物和1个去甲基代谢物,在尿液中没有检测到相关代谢物。结论 20(R)-25-OCH3-PPD在大鼠体内代谢广泛。
Objective To identify the metabolites of the antitumor drug 20 (R) -25-OCH3-PPD in rats. Methods The rats were given intragastric administration of 20 (R) -25-OCH3-PPD (40 mg · kg-1) once a day to collect feces, urine and plasma. The metabolites in feces, urine and plasma of rats were analyzed and identified by LC-MS / MS with MRM and Full Scan MS2. Results Prototyping drugs and 8 metabolites were detected in feces, prototype drugs and 1 demethylated metabolite were detected in plasma, and no relevant metabolites were detected in urine. Conclusion 20 (R) -25-OCH3-PPD is widely metabolized in rats.