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目的 介绍一种从总RNA制备DIG标记的非放射性cDNA探针的方法。方法 以总RNA的反转录产物为模板 ,用随机引物延伸法掺入DIG 11 dUTP来制备cDNA探针。结果 该方法标记的探针灵敏度较高 ,可达 0 .0 1pg。结论 这种cDNA探针可用于差异筛选的反向杂交及Northern杂交。
Objective To introduce a method for preparing DIG-labeled non-radioactive cDNA probes from total RNA. Methods cDNA probes were prepared by reverse transcription of total RNA as a template and by random primer extension into DIG 11 dUTP. Results The method of labeling the probe with high sensitivity, up to 0.01 pg. Conclusion This cDNA probe can be used for differential screening of reverse hybridization and Northern hybridization.