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目的制备D11S4463基因座等位基因分型标准物,并调查该基因座在中国汉族人群中的遗传多态性。方法设计引物,用荧光引物PCR扩增和ABI 3130XL遗传分析仪电泳的方法,对中国汉族520份无关个体血斑样本D11S4463基因座遗传多态性进行调查,用分子克隆的方法构建其等位基因分型标准物。结果 D11S4463基因座在中国汉族人群中共检测出9个等位基因,杂合度、匹配概率、个体识别能力、多态性信息含量及非父排除概率分别为0.735、0.089、0.911、0.73、0.485。应用分子克隆的方法成功构建其等位基因分型标准物,按国际法庭血液遗传学学会推荐的原则命名。结论 D11S4463基因座具有较高的遗传多态性,应用分子克隆的方法构建其等位基因分型标准物,在法医科学实践中具有较高的应用价值。
Objective To prepare allelic ladder alleles of D11S4463 locus and to investigate the genetic polymorphism of the locus in Chinese Han population. Methods The primers were designed and the genetic polymorphisms of D11S4463 locus in 520 Chinese unrelated individuals were investigated by PCR amplification of fluorescent primers and ABI 3130XL genetic analyzer. The alleles were constructed by molecular cloning Parting standards. Results Nine alleles were detected in D11S4463 locus in Chinese Han population. The heterozygosity, matching probability, individual identification ability, polymorphism information content and non-parent exclusion probability were 0.735,0.089,0.911,0.73,0.485 respectively. The method of molecular cloning was used to successfully construct its allelic typing standard, which was named according to the principles recommended by the International Society of Hematological Genes. Conclusion The D11S4463 locus has a high genetic polymorphism. Molecular cloning method is used to construct its allelic typing standard, which has high value in forensic science practice.